genomic organization of leishmania species

نویسندگان

b kazemi department of biotechnology, school of medicine, shahid beheshti university of medical sciences, tehran, iran and cellular and molecular biology research center, shahid beheshti university of medical sciences, tehran, iran

چکیده

leishmania is a protozoan parasite belonging to the family trypanosomatidae, which is found among 88 different countries. the parasite lives as an amastigote in vertebrate macro­phages and as a promastigote in the digestive tract of sand fly. it can be cultured in the laboratory us­ing appropriate culture media. although the sexual cycle of leishmania has not been observed during the promastigote and amastigote stages, it has been reported by some researchers. leishma­nia has eukaryotic cell organization. cell culture is convenient and cost effective, and because posttranslational modifications are common processes in the cultured cells, the cells are used as hosts for preparing eukaryotic recombinant proteins for research. several transcripts of rdna in the leishmania genome are suitable regions for conducting gene transfer. old world leishmania spp. has 36 chromosomes, while new world leishmania spp. has 34 or 35 chromo­somes. the genomic organization and parasitic characteristics have been investigated. leishmania spp. has a unique genomic organization among eukaryotes; the genes do not have introns, and the chromosomes are smaller with larger numbers of genes confined to a smaller space within the nucleus. leishmania spp. genes are organized on one or both dna strands and are transcribed as polycistronic (prokaryotic-like) transcripts from undefined promoters. regulation of gene expres­sion in the members of trypanosomatidae differs from that in other eukaryotes. the trans-splic­ing phenomenon is a necessary step for mrna processing in lower eukaryotes and is observed in leishmania spp. another particular feature of rna editing in leishmania spp. is that mitochon­drial genes encoding respiratory enzymes are edited and transcribed. this review will discuss the chromosomal and mitochondrial (kinetoplast) genomes of leishmania spp. as well as the phenome­non of rna editing in the kinetoplast genome.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Genomic Organization of Leishmania Species

Leishmania is a protozoan parasite belonging to the family Trypanosomatidae, which is found among 88 different countries. The parasite lives as an amastigote in vertebrate macrophages and as a promastigote in the digestive tract of sand fly. It can be cultured in the laboratory using appropriate culture media. Although the sexual cycle of Leishmania has not been observed during the promastigote...

متن کامل

Genomic organization and gene function in Leishmania.

Sequencing of the Leishmania major Friedlin genome is well underway with chromosome 1 (Chr1) and Chr3 having been completely sequenced, and Chr4 virtually complete. Sequencing of several other chromosomes is in progress and the complete genome sequence may be available as soon as 2003. A large proportion ( approximately 70%) of the newly identified genes remains unclassified, with many of these...

متن کامل

The genomic organization of plant pathogenicity in Fusarium species.

Comparative genomics is a powerful tool to infer the molecular basis of fungal pathogenicity and its evolution by identifying differences in gene content and genomic organization between fungi with different hosts or modes of infection. Through comparative analysis, pathogenicity-related chromosomes have been identified in Fusarium oxysporum and Fusarium solani that contain genes for host-speci...

متن کامل

Biochemistry of the Leishmania species.

Since 1980, there has been a burst of published reports describing the biochemistry of various human parasites. Interest in the biochemistry and molecular biology of the human parasites has increased greatly, in part because of the growing awareness of the morbidity and mortality they cause worldwide, especially in developing countries, and because of increased support for parasite research in ...

متن کامل

Genomic organization and expression of the expanded SCG/L/R gene family of Leishmania major: internal clusters and telomeric localization of SCGs mediating species-specific LPG modifications.

Stage-specific modifications to the abundant surface lipophosphoglycan (LPG) adhesin of Leishmania play critical roles in binding and release of the parasite during its infectious cycle in the sand fly, and control the ability of different fly species to transmit different parasite strains and species. In Leishmania major Friedlin V1, binding to a sand fly midgut lectin is mediated by side chai...

متن کامل

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید


عنوان ژورنال:
iranian journal of parasitology

جلد ۶، شماره ۳، صفحات ۱-۱۸

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023